It was established in 1839 at the crossing of the Goulburn River on the Melbourne-Sydney route (now known as the Hume Highway) where an inn was first built. The railway arrived in 1872 along with substantial infrastructure to support it establishing the town as an important rail hub for the Goulburn Valley, the Melbourne-Sydney rail line and North Eastern Victoria.
Seymour is headquarters to a substantial police division which maintains a large Traffic Manangement Unit (Responsible for an area up to 100 km radius of the township) and Crime Investigation Unit. Other important sectors of employment in Seymour includes retail, light engineering, agricultural services support, medical services, and education.
The township services the surrounding agricultural industries (primarily sheep, cattle and wine) as well as the nearby military base of Puckapunyal (Population 1,176) which is an important training centre for the Australian Army.
Seymour hosts an annual Alternative Farming Exposition each February which draws many thousands of visitors. The township is also home to a well established railway museum and several wineries.
Seymour offers a wide selection dining options to visitors that include The Prince of Wales Hotel, The Somerset Winery, The Old Post Office Restaurant, The Seymour Club, The Royal Hotel, and The Railway Hotel which all provide visitors with a selection of fresh locally produced foods and wines.